anova followed by fisher’s lsd test  (GraphPad Software Inc)

Journal: Frontiers in Behavioral Neuroscience

Article Title: Distinct sex differences in ethanol consumption and operant self-administration in C57BL/6J mice with uniform regulation by glutamate AMPAR activity

doi: 10.3389/fnbeh.2024.1498201

Figure Lengend Snippet: Operant EtOH self-administration and progressive ratio testing in male and female mice. (A) Timeline EtOH self-administration procedure showing 3 experimental phases of acquisition, baseline, and progressive ratio testing as a function of week. (B,E) Total number of EtOH reinforced responses (B) and EtOH intake (E) by each mouse during four daily 16 h acquisition sessions, separated by sex. * - indicates significant mean difference between day 4 and day 1, Šídák’s multiple comparisons test, p = 0.007. (C,F) EtOH reinforced responses plotted as a function of baseline day. Black lines are linear regression lines derived from regression analysis showing that the female slopes were significantly different from zero and that the two lines were different from each other for both responses (C) and intake (F) (see text). (D,G) Average EtOH reinforced responses (D) and EtOH intake (G) from week 1 and 4 showing an escalating pattern in female mice and difference between sexes during week 4. * p = 0.05; ** p < 0.01, Fisher’s LSD multiple comparisons test. Progressive ratio breakpoint (H) , duration of progressive ratio session (I) , and headpokes occurring during each session (J) plotted as a function of sex. Data were analyzed via unpaired t -test, * indicates p < 0.05. All data are plotted as MEAN ± SEM.

Article Snippet: Post-hoc comparisons to evaluate specific differences were conducted using Šídák’s multiple comparisons test to control familywise error rates, or Fisher’s LSD test when conducting 4-family pairwise comparisons as implemented in GraphPad Prism.

Techniques: Derivative Assay